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M-MLV Reverse Transcriptase

M-MLV Reverse Transcriptase
M-MLV Reverse Transcriptase

M-MLV Reverse Transcriptase
货号:28025013【28025-013】
单位规格:40000 units

产品型号:28025013

联系方式

86-021-65672052

产品先容

Invitrogen试剂28025013现货供应

M-MLV Reverse Transcriptase
货号:28025013【28025-013】
单位规格:40000 units

产品报价:2161元 【折扣价请咨询!】

产品描述 M-MLV Reverse Transcriptase 现货供应

描述

M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RT) is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. Compared to AMV RT, M-MLV RT lacks DNA endonuclease activity and has a lower RNase H activity.

• Thermostability – Optimal activity at 37°C
• Size of cDNA – MMLV can be used to synthesize first-strand cDNA up to 7 kb
• Applications – Synthesis of first-strand cDNA (1), primer extension (2), sequencing dsDNA (3), cDNA libraries, and RT-PCR (4)

Source: Purified from E. coli expressing the pol gene of M-MLV on a plasmid (5).

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product.

Unit Definition: One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (6).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl2, 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18, 0.1 mg/ml BSA, and enzyme in 50 µl for 10 min. at 37°C (6).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

规格

Optimal Reaction Temperature:37° C
Final Product Size(s):7 kb or less
Reverse Transcriptase:
Ribonuclease H Activity:Reduced
Format:Tube(s)
Template:RNA-DNA Hybrid, ssRNA, ssDNA
Sensitivity:Low
Product Size:40,000 units
Concentration:200 U⁄µl
Enzyme Function:RNA dependent DNA Polymerase
Shipping Condition:Wet Ice
Number of Reactions:200 Reactions

内容及储存

M-MLV RT is supplied with a vial of 5X first strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2 ], and a vial of 100 mM DTT. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Invitrogen试剂28025013现货供应


货号:28025013【28025-013】
单位规格:40000 units

产品报价:2161元 【折扣价请咨询!】

产品相关关键字: Invitrogen试剂现货 gibco试剂现货 逆转录酶现货 反转录酶现货 生物试剂

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