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12800-017DMEM高糖培养基

12800-017DMEM高糖培养基

简要描述:12800-017DMEM高糖培养基英文名称:DMEM, Powder, High Glucose, Pyruvate规格:10 x 1 L性状:干粉培养基货号:12800-017

产品型号:

所属分类:12800-017DMEM高糖培养基

更新时间:2016-04-08

厂商性质:经销商

详情先容

英文名称:DMEM, Powder, High Glucose, Pyruvate
规格:10 x 1 L
性状:干粉培养基
货号:12800-017

12800-017DMEM高糖培养基描述

Dulbeccos Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of Gibco? DMEM modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.

This DMEM is modified as follows:

With Without
• High Glucose • HEPES
• L-glutamine • Sodium Bicarbonate
• Phenol Red  
• Sodium Pyruvate  


The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagles Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco? DMEM is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco? DMEM product made in our Scotland facility (12800-116). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco? cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

12800-017DMEM高糖培养基规格

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
Glucose: High Glucose
Serum Supplementation: Standard Serum Supplementation
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Product Size: 10 x 1 L
Sodium Bicarbonate Buffer: No Sodium Bicarbonate
Shipping Condition: Room Temperature

培养基配方

12800 - DMEM, powder, high glucose, pyruvate

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